Journal: Biochemical pharmacology
Article Title: Characterization of [ 3 H]AZ12464237 as a high affinity, non-nucleotide antagonist radioligand for the P2Y 12 receptor.
doi: 10.1016/j.bcp.2025.116900
Figure Lengend Snippet: Fig. 2. Analytical HPLC chromatogram of [3H]AZ12464237, co-injected with non-radioactive reference compound (AZ12464237). HPLC conditions: Platinum C18 100A 5 μm (250 × 4.6 mm) column using MeCN/H2O/TFA (45:55:0.1, v/v/v) as eluent with UV detection at 254 nm. Eluent flow rate was 1 mL/min and scintillation liquid flow rate was 0.3 mL/min. Retention time = 7.06 min; RCP = 99%.
Article Snippet: Chromatograms were analyzed using the Laura software (version 4.2.10.111, LabLogic Systems Ltd, Sheffield, UK); (2) on an Agilent 1200 series system (Agilent, Santa Clara, CA, US) connected to a Jasco PU2080 Plus Intelligent HPLC pump (Jasco, de Meern, The Netherlands), a Jasco UV-2075 Plus UV/vis detector (Jasco, de Meern, The Netherlands), and a LabLogic radio-HPLC detector β-ram Model 4 (LabLogic Systems Ltd, Sheffield, UK).
Techniques: Injection